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KMID : 1044820180480010060
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Journal of Periodontal & Implant Science
2018 Volume.48 No. 1 p.60 ~ p.68
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Porphyromonas gingivalis accelerates atherosclerosis through oxidation of high-density lipoprotein
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Kim Hyun-Joo
Cha Gil-Sun
Kim Hyung-Joon
Kwon Eun-Young
Lee Ju-Youn
Choi Jeom-Il
Joo Ji-Young
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Abstract
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Purpose: The aim of this study was to evaluate the ability of Porphyromonas gingivalis (P. gingivalis) to induce oxidation of high-density lipoprotein (HDL) and to determine whether the oxidized HDL induced by P. gingivalis exhibited altered antiatherogenic function or became proatherogenic.
Methods: P. gingivalis and THP-1 monocytes were cultured, and the extent of HDL oxidation induced by P. gingivalis was evaluated by a thiobarbituric acid-reactive substances (TBARS) assay. To evaluate the altered antiatherogenic and proatherogenic properties of P. gingivalis-treated HDL, lipid oxidation was quantified by the TBARS assay, and tumor necrosis factor alpha (TNF-α) levels and the gelatinolytic activity of matrix metalloproteinase (MMP)-9 were also measured. After incubating macrophages with HDL and P. gingivalis, Oil Red O staining was performed to examine foam cells.
Results: P. gingivalis induced HDL oxidation. The HDL treated by P. gingivalis did not reduce lipid oxidation and may have enhanced the formation of MMP-9 and TNF-α. P. gingivalis-treated macrophages exhibited more lipid aggregates than untreated macrophages.
Conclusions: P. gingivalis induced HDL oxidation, impairing the atheroprotective function of HDL and making it proatherogenic by eliciting a proinflammatory response through its interaction with monocytes/macrophages.
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KEYWORD
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Atherosclerosis, Cardiovascular diseases, Cholesterol, Periodontitis, Porphyromonas gingivalis
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